Properties of aspartate racemase, a pyridoxal 5'-phosphate-independent amino acid racemase.
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چکیده
منابع مشابه
A novel pyridoxal 5'-phosphate-dependent amino acid racemase in the Aplysia californica central nervous system.
D-aspartate (D-Asp) is found in specific neurons, transported to neuronal terminals and released in a stimulation-dependent manner. Because D-Asp formation is not well understood, determining its function has proved challenging. Significant levels of D-Asp are present in the cerebral ganglion of the F- and C-clusters of the invertebrate Aplysia californica, and D-Asp appears to be involved in c...
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An open reading frame of 1100 bp in the partially sequenced genome sequence of alkaliphilic Bacillus pseudofirmus OF4 was identified as a putative alanine racemase gene (dadX(OF4)), which was cloned and expressed in Escherichia coli BL21 (DE3). The encoded protein DadX(OF4) was purified to homogeneity by His(6)-tag affinity column, gel filtration and ion-exchange chromatography. The amino acid ...
متن کاملAspartate racemase, generating neuronal D-aspartate, regulates adult neurogenesis.
D-aspartic acid is abundant in the developing brain. We have identified and cloned mammalian aspartate racemase (DR), which converts L-aspartate to D-aspartate and colocalizes with D-aspartate in the brain and neuroendocrine tissues. Depletion of DR by retrovirus-mediated expression of short-hairpin RNA in newborn neurons of the adult hippocampus elicits profound defects in the dendritic develo...
متن کاملTransfer of pyridoxal 5'-phosphate from albumin-pyridoxal 5'-phosphate complex to apo-aspartate aminotransferase.
Pyridoxal 5•L-phosphate (PLP) is known to combine with bovine serum albumin to form a(1:1) complex which scarcely dissociates, even when subjected to intensive dialysis. When this complex was incubated with apo-aspartate aminotransferase (apoGOT) for an appropriate time and the preincubated mixture then submitted to the usual GOT assay, the appearance of GOT activity was obviously confirmed, in...
متن کاملGlutamic acid racemase from Lactobacillus arabinosus.
The existence of a glutamic acid racemase activity in Lactobacillus arabinosus has been reported by Narrod and Wood (1) and by Ayengar and Roberts (2). As has been pointed out by Meister (3) these observations with dried cell preparations would not exclude a mechanism for glutamic acid racemization in which alanine racemase is coupled with n-glutamic acid-nalanine transaminase and n-glutamic ac...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)36969-8